Alkaline buffers release EDRF from bovine cultured aortic endothelial cells
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چکیده
منابع مشابه
Cultured Bovine Aortic Endothelial and Smooth Muscle Cells
Endothelial cell (EC) injury and the response of EC and smooth muscle cells (SMCs) to injury contribute to the pathophysiology in patients with vascular disease and atherosclerosis. Since platelets have been suggested to play an important role in modulating vascular injury, the present study was undertaken to examine the influence and mechanism of action of individual platelet factors on bovine...
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Cultured bovine aortic endothelial cells were examined for renin activity by biochemical, immunological, and immunohistochemical techniques. When cell sonicates were incubated with renin substrate, linear generation of angiotensin I was observed (1.12 ± 0.2 ng angiotensin I/10 cells per hr). The effect of pH on this activity was similar to that of bovine renal renin, and renin antibodies inhibi...
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The extracellular matrix (ECM) of cultured bovine aortic endothelial cells (BAEs) was analyzed by immunoblotting and reverse fibrin autography and shown to contain type 1 plasminogen activator inhibitor (PAl-i ). Most PAl-i in the ECM formed complexes with exogenously added tissuetype plasminogen activator (tPA). demonstrating that this PAl-i was functionally active. The resulting tPA/PAI-1 com...
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The role of extracellular ethanolamine in phospholipid synthesis was examined in cultured bovine aortic endothelial cells. Serine and ethanolamine were both readily accumulated by these cells and incorporated into phospholipid. Exposing cells to extracellular ethanolamine for 4-6 weeks had no effect on cell growth, yet increased the phosphatidylethanolamine content of these cells by 31% as comp...
متن کاملBovine Aortic Endothelial Cells
As bstract. We examined the effect of bovine aortic endothelial cell culture supernatants upon the generation of procoagulant activity by human blood monocytes. Confluent endothelial monolayers were cultured for up to 96 h. At timed intervals, culture supernatants were collected and incubated for 5 h with lipopolysaccharide-stimulated human peripheral blood mononuclear cells. The procoagulant a...
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ژورنال
عنوان ژورنال: British Journal of Pharmacology
سال: 1991
ISSN: 0007-1188
DOI: 10.1111/j.1476-5381.1991.tb09783.x